Stress caused by sleep deprival: liver morphometric analysis

Main Article Content

Marília Capuço Oliveira
Neide Aparecida Micelli Domingos
Alex Tadeu Martins
Reinaldo Azoubel
Maria Cristina de Oliveira Santos Miyazaki
Rodrigo Estevan Imperial
Milton Dorival Pires
Moacir Fernandes de Godoy

Abstract

Objective: To evaluate the effect of stress induced by sleep deprivation on nuclear and cellular features of rat liver. Methods: 16 adult male Wistar rats (200–260 g) in controlled light cycle received diet with customary salt quantity and free access to water and food. The animals were divided into two groups with 8 animals each (experimental group and control group). Animals stayed in the same box (two at a time) during seven days and after that period they were weighted e separated. The control group animal continued in the same box and the experimental group animal were transferred to a sleep deprivation apparatus. After the 96 hours the animals were sacrificed by an excessive anesthetic dose; animals were weighted and their liver extracted. Results: There was no significant difference between experimental and control group regarding weight, although there was a decrease of weight on the experiment group. Karyotipical analysis showed significant smaller diameter (p=0.03) and volume (p=0.044) of hepatocyte nuclei in experimental group. Stereological analyses showed significant differences in experimental group for nucleus (larger, p=0.036), cytoplasm (smaller, p=0.009) and other structures (larger, p=0.008). Conclusion: Stress seems to contribute to alteration of hepatic cell structure.

Downloads

Download data is not yet available.

Article Details

How to Cite
Oliveira, M. C., Domingos, N. A. M., Martins, A. T., Azoubel, R., Miyazaki, M. C. de O. S., Imperial, R. E., Pires, M. D., & de Godoy, M. F. (2013). Stress caused by sleep deprival: liver morphometric analysis. ABCS Health Sciences, 38(2). https://doi.org/10.7322/abcshs.v38i2.11
Section
Original Articles

References

1. Lipp MEN, Malagris LEN. Estresse: aspectos históricos, teóricos e clínicos. In: Rangé B (org.). Psicoterapias cognitivo-comportamentais: um diálogo com a psiquiatria. Porto Alegre: Artmed; 2011.

2. Greenberg JS. Administração do estresse. 6ªed. Barueri: Manole; 2002.

3. Sapolsky RM, Romero LM, Munck AU. How do glucocorticoids influence stress responses? Integranting permissive, suppressive, stimulatory and preparative actions. Endocr Rev. 2000; 21(1):55-89.

4. Guyton H. Tratado de fisiologia médica. Elsevier; 2006.

5. Jun J, Polotsky VY. Metabolic consequences of sleep-disordered breathing. ILAR J 2009;50(3):289-306. http://dx.doi.org/10.1093/ilar.50.3.289

6. Khani S, Tayek JA. Cortisol increases gluconeogenesis in humans: its role in metabolic syndrome. Clin Sci (Lond). 2001;101(6):739-47. http://dx.doi.org/10.1042/CS20010180

7. Björntörp P. Metabolic difference between visceral fat and subcutaneous abdominal fat. Diabetes Metab. 2000;26(suppl 3):10-12.

8. Goldstein RE, Wasserman DH, McGuinness OP, Brooks D, Lacy DB, Cherrington AD. Effects of chronic elevation in plasma cortisol on hepatic carbohydrate metabolism. Am J Physiol. 1993;264(1 pt 1): E119-27.

9. Palma BD, Tiba PA, Machado RB, Tufik S, Suchecki D. Repercussões imunológicas dos distúrbios do sono: o eixo hipotálamo-pituitária-adrenal como fator modulador. Rev Bras Psiquiatr. 2007;29(Supl 1):S33-8.

10. Andersen ML, Martins PJF, D’Almeida V, Bignotto M, Tufik S. Endocrinological and catecholaminergic alterations during sleep deprivation and recovery in male rats. J Sleel Res. 2005;14(1):83-90. http://dx.doi.org/10.1111/j.1365-2869.2004.00428.x

11. Knustson KL, Spiegel K, Penev P, Van E. The metabolic consequences of sleep deprivation. Sleep Med Ver. 2007;11(3):163-178. http://dx.doi.org/10.1016/j.smrv.2007.01.002

12. De Mattos AB, Pinto MJ, Oliveira C, Biz C, Ribeiro EB, Nascimento CM, et al. Dietary fish oil did not prevent sleep deprived rats from a reduction in adipose tissue adinonectin gene expression. Lipids Health Dis. 2008; 7: 43. http://dx.doi.org/10.1186/1476-511X-7-43

13. Chang HM, Wu UI, Lin TB, Lan CT, Chien WC, Huang WL, et al. Total sleep deprivation inhibits the neuronal nitric oxide synthase and cytochrome oxidase reactivities in the nodose ganglion of adult rats. J Anat. 2006;209(2):239-250. http://dx.doi.org/10.1111/j.1469-7580.2006.00594.x

14. Thase ME. Depression and sleep: pathophysiology and treatment. Dialogues Clin Neurosci. 2006;8(2):217-26.

15. Vgontzas AN, Chrousos GP. Sleep, the hypothalamic-pituitary-adrenal axis, and cytokines: multiple interactions and disturbances in sleeo disorders. Endocrinol Metab Clin North Am. 2002;31(1):15-36. http://dx.doi.org/10.1016/S0889-8529(01)00005-6

16. Bueno OF, Oliveira MG, Lobo LL, Morais PR, Melo FH, Tufik S. Cholinergic modulation of inhbitory avoidance impairment induced by paradoxical sleep deprivation. Prog Neuropsychopharmacol Biol Psychiatry. 2000;24(4):595-606. http://dx.doi.org/10.1016/S0278-5846(00)00095-6

17. Ferraz MR, Ferraz MM, Santos R. How REM sleep deprivation and amantadine affects male rat sexual behavior. Pharmacol Biochem Behav. 2001; 69(3-4):325-332. http://dx.doi.org/10.1016/S0091-3057(01)00508-1

18. Hipólide DC, Suchecki D, Pimentel de Carvalho Pinto A, Faria EC, Tufik S, Luz J. Paradoxical sleep deprivation and sleel recovery: effects on the hypothalamic-pituitary-adrenal axis activity, energy balance and body composition of rats. J Neuroendocrinol. 2006;18(4):231-38. http://dx.doi.org/10.1111/j.1365-2826.2006.01412.x

19. Koban M, Sita LV, Le WW, Hoffman GE. Sleep deprivation of rats: the hyperphagic response is real. Sleep. 2008;31(7):972-33.

20. Galli MR, Martins PJF, Souza FG, Azzalis LA, D´Almeida V, Tufik S. Efeitos da privação de sono sobre o metabolismo hepático de ratos. Disponível em http://www.unifesp.br/prograd/pibic2003/Resumos/25.prn.pdf 2003. Acesso em: 17 jun. 2013.

21. Moles A, Bartolomucci A, Garbugino L, Conti R, Caprioli A, Coccurello R, et al. Psychosocial stress affects energy balance in mice: modulation by social status. Psychoneuroendocrinology. 2006;31(5):623-633. http://dx.doi.org/10.1016/j.psyneuen.2006.01.004

22. Chang HM, Mai FD, Chen BJ, Wu Un-In, Huang YL, Lan CT, et al. Sleep deprivation predisposes liver to oxidative stress and phospholipid damage: a quantitative molecular imaging study. J Anat. 2008;12(3):295-305. http://dx.doi.org/10.1111/j.1469-7580.2008.00860.x

23. Meyer TN, Silva AL. Resposta celular ao estresse. Rev Assoc Med Bras. 1999;45(2):181-188. http://dx.doi.org/10.1590/S0104-42301999000200016

24. Berra CM, Menck CFM, Di Mascio P. Estresse oxidativo, lesões no genoma e processos de sinalização no controle do ciclo celular. Rev Quím Nova. 2006;29(6): 1340-1344. http://dx.doi.org/10.1590/S0100-40422006000600032