High energy photobiomodulation therapy in the early days of injury improves sciatic nerve regeneration in mice

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Luana Gabriel de Souza
Ramon Bauer Cardoso
Heloyse Uliam Kuriki
Alexandre Márcio Marcolino
Marisa de Cássia Registro Fonseca
Rafael Inácio Barbosa


Introduction: Different studies have evaluated the effects of electrophysical agents on regeneration after peripheral nerve injury. Among them, the most used in clinical and experimental research is photobiomodulation therapy (PBMT). Objective: To analyze the effect of standard energy (16.8 J) of PBMT on peripheral nerve regeneration, applied at different periods after sciatic nerve injury in mice. Methods: Thirty male Swiss mice were divided into six groups: naive; sham; control; LLLT-01 (660 nm, 16.8 J of total energy emitted in 1 day); LLLT-04 (660 nm, 4.2 J per day, 16.8 J of total energy emitted in 4 days); LLLT-28, (660 nm, 0.6 J per day, 16.8 J of total energy emitted over 28 days). The animals were evaluated using thermal hyperalgesia, Sciatic Functional Index (SFI), and Static Sciatic Index (SSI). Data were obtained at baseline and after 7, 14, 21, and 28 days after surgery. Results: For the SFI and SSI, all groups showed significant differences compared to the control group, and the LLLT-04 group presented the best results among those receiving PBMT. In the assessment of thermal hyperalgesia, there was a significant difference in the 14th day of evaluation in the LLLT-04 group. Conclusion: The application of 16.8 J was useful in sciatic nerve regeneration with an improvement of hyperalgesia, with higher efficacy when applied in four days (4.2 J/day).


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Souza, L. G. de, Cardoso, R. B., Kuriki, H. U., Marcolino, A. M., Fonseca, M. de C. R., & Barbosa, R. I. (2020). High energy photobiomodulation therapy in the early days of injury improves sciatic nerve regeneration in mice. ABCS Health Sciences, 45, e020016. https://doi.org/10.7322/abcshs.45.2020.1345
Original Articles
Author Biography

Rafael Inácio Barbosa, Programa de Pós-Graduação em Ciências da Reabilitação, Universidade Federal de Santa Catarina (UFSC) – Araranguá (SC), Brazil

Professor Adjunto do Curso de Fisioterapia da Universidade Federal de Santa Catarina, UFSC/Araranguá e Docente Permanente do Programa de Mestrado em Ciências da Reabilitação da UFSC-Araranguá. Pós-doutorado, em andamento, pelo pelo programa de Reabilitação e Desempenho Funcional (PPGRDF) na Faculdade de Medicina de Ribeirão Preto/Universidade de São Paulo; Doutorado pelo PPGRDF/Universidade de São Paulo - FMRP/USP; Mestre em Ciências da Saúde - Opção: Reabilitação pela FMRP/USP; Aprimoramento Profissional em Fisioterapia Ortopédica e Traumatológica pelo Hospital das Clínicas da FMRP/USP e Pós-graduação em Reabilitação do Membro Superior pela Universidade Federal de São Carlos. Atualmente é Presidente Nacional da ABRAFITO; Revisor dos periódicos: Trials, American Journal of Physical Medicine and Rehabilitation, Rheumatology International, Lasers in Medical Science, Lasers in Surgery & Medicine, Brazilian Journal of Physical Therapy e Fisioterapia e Pesquisa. Tem experiência clínica e acadêmica na área de Fisioterapia em Ortopedia e Traumatologia e Fotobiomodulação. Membro dos Grupos de Pesquisa: Avaliação e Reabilitação do Aparelho Locomotor - UFSC, Laboratório de Recursos Fisioterapêuticos - LARF/USP e Avaliação e Intervenção Fisioterapêutica nas Disfunções da Mão e Membro Superior - USP/RP.


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