Evaluation of the comparative effects of infrared and red laser photobiomodulation therapy on skeletal muscle atrophy in an immobilization model in rats

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Silma Rodrigues Gonçalves
Carla Roberta Tim
Nivaldo Antonio Parizotto
Cintia Cristina Santi Martignago
Marcelo Cavenaghi Pereira da Silva
Carlos Alberto Anaruma
Hananiah Tardivo Quintana
Lívia Assis


Introduction: Skeletal muscle atrophy leads to a reduction in muscle strength, functionality and the quality of life of individuals. Objective: To explore the effects of two different wavelengths (red and infrared) of laser PBMT on muscle atrophy and its active ingredients on skeletal muscle atrophy using an in vivo model of muscle atrophy. Methods: Thirty-two Wistar rats were randomly divided into four experimental groups: control (CG) animals were not immobilized and did not receive any type of treatment; immobilized animals with no treatment (ImC); immobilized animals submitted to red laser with wavelength of 660 nm (ImR) and near-infrared laser with wavelength of 808 nm (ImIR) treatments. Treatments were applied daily, at 2 points in the right gastrocnemius muscle (cranial and caudal), through the punctual contact technique, for 9 sessions, with the first application immediately after removing the cast. Results: The histological results demonstrated that in both treated groups (red and infrared wavelengths) a reduction of the inflammatory infiltrate and less connective tissue thickening when compared to the ImC. However, only infrared light was observed regenerating muscle fibers and an increase in the number of oxidative fibers (type I). Conclusion: These results suggest that red and infrared wavelength laser PBMT were able to promote changes in the morphology of the gastrocnemius muscle submitted to atrophy in an experimental immobilization model, reducing the inflammatory infiltrate and the formation of intramuscular connective tissue. However, infrared laser PBMT promoted more evident positive effects by increasing regenerating muscle fibers and the number of oxidative fibers.


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Gonçalves, S. R., Tim, C. R., Parizotto, N. A., Martignago, C. C. S., Silva, M. C. P. da, Anaruma, C. A., Quintana, H. T., & Assis, L. (2023). Evaluation of the comparative effects of infrared and red laser photobiomodulation therapy on skeletal muscle atrophy in an immobilization model in rats. ABCS Health Sciences, 48, e023232. https://doi.org/10.7322/abcshs.2021248.1964
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