Molecular Detection of Mycoplasma hominis in Cervicovaginal Samples by qPCR
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Abstract
Introduction: Mycoplasma hominis is one of the microorganisms associated with vaginal microbiota dysbiosis and infertility, and its prevention and investigation are important criteria for monitoring gynecological health during the reproductive age. Objective: To determine the prevalence of M. hominis in the cervicovaginal samples of women receiving care through the Unified Health System (SUS) and relate it to sociodemographic, behavioral, and clinical characteristics. Methods: Cross-sectional study carried out in a Gynecology Outpatient Clinic. The research for M. hominis was performed using the Real-Time Polymerase Chain Reaction (qPCR) technique in samples of cervicovaginal samples of women undergoing the Pap smear. Sociodemographic and clinical data were collected through a questionnaire, and such information was related to the chi-square (χ²) test or Fisher's exact test, with a significance of 5%. Results: A total of 182 women were included, with a mean age of 43.1 ± 10.9 years, White (64.8%), with a predominance of ≤4 years of schooling (46.7%), and with paid work (59.3%). The positivity for M. hominis in the samples was 7.7%. The presence of the microorganism studied was positively related to having had a Pap smear more than 36 months ago (p<0.05). Conclusion: The women of reproductive age who did not undergo cervical cancer screening in the last 3 years should be considered when monitoring genital health and identifying possible pathogens that may participate in microbiota dysbiosis.
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