Recommendations for specimen collection for detection and diagnosis of COVID-19

Main Article Content

Juliana Dall'Agnol
Eda Schwartz
Fernanda Lise

Abstract

Introduction: The spread of SARS-CoV-2 has caused a global public health crisis (pandemic). One of the most important measures to control the transmission chain of the new coronavirus is to identify those infected through laboratory testing. Objective: Synthesize the recommendations for the specimen collection for detection and diagnosis of COVID-19. Methods: This is an integrative review, considering the publications of the following databases: PubMed and Google Academic from January 2020. Results: 468 publications were identified, 20 of which were considered eligible. The publications recommend that technical training for specimen collection and careful observation of infection prevention protocols are fundamental. This manuscript highlights the steps for specimen collection as materials for collection, storage, transportation, individual protection, and laboratory analysis of samples. Currently, the Reverse Transcription - Polymerase Chain Reaction test is the recommended and gold standard method of identifying COVID-19 cases. Serological tests play an important role in research and surveillance. Conclusion: In summary, the documents ensure that the RT-PCR is the gold standard for SARS-CoV-2 detection and recommend standardization of collection and conditioning methods to avoid errors related to the collection and false negative results.

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How to Cite
Dall’Agnol, J., Schwartz, E., & Lise, F. (2021). Recommendations for specimen collection for detection and diagnosis of COVID-19. ABCS Health Sciences, 46, e021302. https://doi.org/10.7322/abcshs.2020195.1642
Section
Review Articles

References

World Health Organization (WHO). Severe acute respiratory syndrome (SARS). Available from: https://www.who.int/health-topics/severe-acute-respiratory-syndrome#tab=tab_1

Worldometers. COVID-19 Coronavirus Pandemic. Available from: https://www.worldometers.info/coronavirus/

World Health Organization (WHO). Laboratory testing for 2019 novel coronavirus (2019-nCOV) in suspected human cases. Available from: https://www.who.int/publications/i/item/laboratory-testing-of-2019-novel-coronavirus-(-2019-ncov)-in-suspected-human-cases-interim-guidance

World Health Organization (WHO). Laboratory testing of human suspected cases of novel coronavirus (nCoV) infection – Interim guidance. Available from: https:// www.who.int/health-topics/coronavirus/laboratory-diagnostics-for-novel-coronavirus

Centers for Disease Control and Prevention (CDC). Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens for Covid-19. Available from: https://www.cdc.gov/coronavirus/2019-nCoV/lab/guidelines-clinical-specimens.html#specimen

Agência Nacional de Vigilância Sanitária (ANVISA). Testes para Covid-19. Available from: http://portal.anvisa.gov.br/documents/219201/4340788/Perguntas+e+respostas+-+testes+para+Covid-19.pdf/9fe182c3-859b-475f-ac9f-7d2a758e48e7

Stanford Health Care. Covid-19 Specimen Collection & Handling: Covid-19 Sars-CoV-2 RT-PCR molecular testing. Available from: https://stanfordhealthcare.org/health-care-professionals/covid-19-test/covid-19-molecular-testing.html

Centers for Disease Control and Prevention (CDC). CDC2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel. Available from: https://www.fda.gov/media/134922/download

Bwire GM, Majigo MV, Njiro BJ, Mawazo A. Detection profile of SARS-CoV-2 using RT-PCR in different types of clinical specimens: A systematic review and meta-analysis. J Med Virol. 2020;1-7. https://doi.org/10.1002/jmv.26349

Castro R, Luz PM, Wakimoto MD, Veloso VG, Grinsztejn B, Perazzo H. COVID-19: a meta-analysis of diagnostic test accuracy of commercial assays registered in Brazil. Braz J Infect Dis. 2020;24(2):180-7. https://doi.org/10.1016/j.bjid.2020.04.003

Adeniji AA. 'Self-collected upper respiratory tract swabs for COVID-19 test': a feasible way to increase overall testing rate and conserve resources in South Africa. Afr J Prim Health Care Fam Med. 2020;12(1):2445. https://doi.org/10.4102/phcfm.v12i1.2445

Siegler AJ, Hall E, Luisi N, Zlotorzynska M, Wilde G, Sanchez T, et al. Willingness to Seek Diagnostic Testing for SARS-CoV-2 With Home, Drive-through, and Clinic-Based Specimen Collection Locations. Open Forum Infect Dis. 2020;7(7):ofaa269. https://doi.org/10.1093/ofid/ofaa269

Associação Nacional de Hospitais Privados (ANAHP). Hospital Israelita Albert Einstein cria primeiro exame genético do mundo para detecção em larga escala do novo coronavírus por meio da técnica de sequenciamento NGS. Available from: https://www.einstein.br/sobre-einstein/imprensa/press-release/einstein-cria-primeiro-teste-genetico-para-deteccao-da-covid19

Mendelics Análise Genômica (MAG). Novo teste de detecção para COVID. Available from: https://www.mendelics.com/assets/PareCovid_Informacoes_Atualizacao1506.pdf

Food and Drug Administration (FDA). Yale School of Public Health. Available from: https://www.fda.gov/media/141194/download.

Agência Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). Pesquisadores da USP desenvolvem teste diagnóstico de COVID-19 pela saliva. Available from: https://agencia.fapesp.br/pesquisadores-da-usp-desenvolvem-teste-de-diagnostico-de-covid-19-pela-saliva/33998/

Parada-Fernández F, Fonseca-Escobar D, Carvajal-Guzmán M, Sepúlveda-Verdugo C. Comparación de la muestra salival y de nasofaringe en la detección de SARS-CoV-2 mediante RT-PCR. Int J Odontostomat. 2020;14(4):540-3. http://dx.doi.org/10.4067/S0718-381X2020000400540

Nagura-Ikeda M, Imai K, Tabata S, Miyoshi K, Murahara N, Mizuno T, et al. Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription-Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19. J Clin Microbiol. 2020;58(9):e01438-20. https://doi.org/10.1128/JCM.01438-20

Whittemore R, Knafl K. The integrative review: updated methodology. J Adv Nurs. 2005;52(5):546-53.

https://doi.org/10.1111/j.1365-2648.2005.03621.x

Brasil. Conselho Nacional de Saúde. Resolução 466/12. Trata de pesquisas em seres humanos e atualiza a resolução 196. Available from: http://conselho.saude.gov.br/resolucoes/2012/Reso466.pdf

Agência Nacional de Vigilância Sanitária (ANVISA). Nota Técnica n 04-2020 GVIMS-GGTES-ANVISA-Atualizada. Available from: http://portal.anvisa.gov.br/documents/33852/271858/Nota+T%C3%A9cnica+n+04-2020+GVIMS-GGTES-ANVISA-ATUALIZADA/ab598660-3de4-4f14-8e6f-b9341c196b28

Bain W, Lee JS, Watson AM, Stitt-Fischer MS. Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and Covid-19 Clinical Specimens. Curr Protoc Cytom. 2020;93(1):e77. https://doi.org/10.1002/cpcy.77

Sociedade Brasileira de Análises Clínicas (SBAC). Métodos laboratoriais para diagnóstico da COVID-19. Available from: https://www.sbac.org.br/blog/2020/03/25/metodos-laboratoriais-para-diagnostico-da-covid-19/

Estado do Rio Grande do Sul. Secretaria da Saúde do Rio Grande do Sul. Centro Estadual de Vigilância Sanitária. Orientações para coleta e transporte de secreção respiratória - 2020. Available from: https://saude.rs.gov.br/upload/arquivos/202002/06115208-2020-orientacoes-coleta-amostra-coronavirus-anexo-plano.pdf

Centers for Disease Control and Prevention (CDC). Collection and Submission of Postmortem Specimens from Deceased Persons with Known or Suspected COVID-19. Available from: https://www.cdc.gov/coronavirus/2019-ncov/hcp/guidance-postmortem-specimens.html

Centers for Disease Control and Prevention (CDC). Specimen collection guidelines. Available from: https://www.cdc.gov/urdo/downloads/SpecCollectionGuidelines.pdf