Polissacarídeo esquizofilan de Schizophyllum commune demonstra efeito antinoceptivo em modelo pré-clínico de dor aguda
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Introdução: Aproximadamente 25% a 29% da população mundial sente dor, que motiva a busca por serviços de emergência. Objetivo: Avaliar o efeito do esquizofilan (SPG), um polissacarídeo β-(1→3),(1→6) de Schizophyllum commune, em modelos pré-clínicos de dor aguda e performance neuromuscular. Métodos: Camundongos Swiss adultos (20-30g, 60 dias) foram aclimatados durante uma semana em grupos de 7 por gaiola antes do experimento. SPG foi administrado, intraperitonealmente, em doses de 0.1, 1.0,0 3.0, 5.0, 10.0, 30.0 e 100.0 mg/Kg para o teste de contorções abdominais, e 1.0, 10.0 e 30.0 mg/Kg para os testes de formalina e rotarod, respectivamente. A análise estatística foi realizada utilizando ANOVA one-way, seguido de post hoc de Duncan, conforme apropriado (p<0.05). Resultados: A respeito do teste de contorções abdominais, SPG nas doses de 1.0, 5.0, 10.0, 30.0 e 100.0 mg/Kg promoveram uma redução significativa na contorção de, respectivamente, 90.6%, 86.6%, 83.0%, 86.6% e 76.2%. No teste de formalina, a dose de SPG de 30 mg/Kg reduziu o tempo de nocicepção de fase II em 78.0%. Sedação relevante foi observada apenas no SPG 100 mg/Kg no teste de Rotarod. Conclusão: SPG demonstrou efeitos analgésicos significativos na dor inflamatória aguda sem causar depressão concomitante do sistema nervoso central.
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Referências
Associação Portuguesa para o Estudo da Dor (APED). Declaração de Montreal. Available from: https://www.aped-dor.org/index.php/informacoes-uteis/declaracao-de-montreal
Abiuso N, Santelices JL, Quezada R. Manejo del dolor agudo en el Servicio de Urgencia. Rev Med Clín Condes. 2017;28(2):248-60. http://doi.org/10.1016/j.rmclc.2017.04.012
Non-steroidal anti-inflammatory drugs (NSAIDs). These medicines are widely used to relieve pain, reduce inflammation, and bring down a high temperature. Available from: https://www.nhs.uk/conditions/nsaids/
Castro EM, Calder PC, Roche HM. β-1,3/1,6-Glucans, and Immunity: State of the Art and Future Directions. Mol Nutr Food Res. 2021;65(1):e1901071. http://doi.org/10.1002/mnfr.201901071
Choi Y, Nguyen HTK, Lee TS, Kim JK, Choi J. Genetic Diversity and Dye-Decolorizing Spectrum of Schizophyllum commune Population. J Microbiol Biotechnol. 2020;30(10):1525-35. http://doi.org/10.4014/jmb.2006.06049
Thongsiri C, Nagai-Yoshioka Y, Yamasaki R, Adachi Y, Usui M, Nakashima K, et al. Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria. Carbohydr Polym. 2021;253:117285. http://doi.org/10.1016/j.carbpol.2020.117285
Wong JH, Ng TB, Chan HHL, Liu Q, Man GCW, Zhang CZ, et al. Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials. Appl Microbiol Biotechnol. 2020;104(11):4675-4703. http://doi.org/10.1007/s00253-020-10476-4
Xie Z, Wang Y, Huang J, Qian N, Shen G, Chen L. Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages. Int J Biol Macromol. 2019;129:61-7. http://doi.org/10.1016/j.ijbiomac.2019.02.023
Valasques Junior GL, Cedro PÉP, Mendes TPS, Miranda ACA, Côrtes Filho AB, Lima DM, et al. Characterization and biological activities of polysaccharides extracted from the filamentous fungal cell wall: An updated literature review. Res Soc Dev. 2020;9(11):e62191110217. http://doi.org/10.33448/rsd-v9i11.10217
Vanin AP, Visentin EZ, Fontana RC, Leal MCBM, Silva SA. Stokke BT, et al. β-(1 → 3) (1 → 6) glucan from Schizophyllum commune 227E.32: High yield production via glucose/xylose co-metabolization. Carbohydr Polym. 2023;320:121176. https://doi.org/10.1016/j.carbpol.2023.121176
Koster R, Anderson M, Beer EJ. Acetic acid-induced analgesic screening. Federation Proceedings. 1959;18:412-17.
Dubuisson D, Dennis SG. The formalin test: A quantitative study of the analgesic effects of morphine, meperidine, and brain stimulation in rats and cats. Pain. 1977;4(2):161-74. https://doi.org/10.1016/0304-3959(77)90130-0
Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain. 1987;30(1):103-14. https://doi.org/10.1016/0304-3959(87)90088-1
Dunham NW, Miya TS. A note on a simple apparatus for detecting neurological deficit in rats and mice. J Am Pharm Assoc. 1957;46(3):208-9. https://doi.org/10.1002/jps.3030460322
Bohlen M, Cameron A, Metten P, Crabbe JC, Wahlsten D. Calibration of rotational acceleration for the Rotarod test of rodent motor coordination. J Neurosc Methods. 2009;178(1):10-4. https://doi.org/10.1016/j.jneumeth.2008.11.001
Rustay NR, Wahlsten D, Crabbe JC. Influence of task parameters on rotarod performance and sensitivity to ethanol in mice. Behav Brain Res. 2003;141(2):237-49. https://doi.org/10.1016/s0166-4328(02)00376-5
Williams M, Roger D. Porsolt RD. CNS Safety Pharmacology. In: xPharm: The Comprehensive Pharmacology Reference. 2007;1-13. https://doi.org/10.1016/B978-008055232-3.63682-7
Siegmund E, Cadmus R, Lu G. A Method for evaluating both non-narcotic and narcotic analgesics. Proc Soc Exp Biol Med. 1957;95(4):729-31. https://doi.org/10.3181/00379727-95-23345
Collier HO, Dinneen LC, Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother. 1968;32(2):295-310. https://doi.org/10.1111/j.1476-5381.1968.tb00973.x
Burke A, Smyth E, Fitzgerald GA. Analgesic-antipyretic agents, pharmacotherapy of gout. In: Brunton LL, Lazo JS, Parker KL. Goodman and Gilman: Pharmacological Bases of therapeutics. 11th edition. New York: McGraw Hill, 2006; p. 671-715.
Tjølsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The formalin test: an evaluation of the method. Pain. 1992;51(1):5-17. https://doi.org/10.1016/0304-3959(92)90003-T
McCall WD, Tanner KD, Levine JD. Formalin induces biphasic activity in C-fibers in the rat. Neurosci Lett. 1996;208(1):45-8. https://doi.org/10.1016/0304-3940(96)12552-0
Saddi GM, Abbott FV. The formalin test in the mouse: A parametric analysis of scoring properties. Pain. 2000;89(1):53-63. https://doi.org/10.1016/S0304-3959(00)00348-1
Fu KY, Light AR, Maixner W. Relationship between nociceptor activity, peripheral edema, spinal microglial activation, and long-term hyperalgesia induced by formalin. Neuroscience. 2000;101(4):1127-35. https://doi.org/10.1016/s0306-4522(00)00376-6
Thongsiri C, Nagai-Yoshioka Y, Yamasaki R, Adachi Y, Usui M, Nakashima K, et al. Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria. Carbohydr Polym. 2021;253;117285. https://doi.org/10.1016/j.carbpol.2020.117285
Bashir KMI, Choi JS. Clinical and Physiological Perspectives of β-Glucans: The Past, Present, and Future. Int J Mol Sci. 2017;18(9):1906. https://doi.org/10.3390/ijms18091906
Baggio CH, Freitas CS, Martins DF, Mazzardo L, Smiderle FR, Sassaki GL, et al. Antinociceptive Effects of (1→3), (1→6)-Linked β-Glucan Isolated From Pleurotus pulmonarius in Models of Acute and Neuropathic Pain in Mice: Evidence for a Role for Glutamatergic Receptors and Cytokine Pathways. J Pain. 2010;11(10):965-71. https://doi.org/10.1016/j.jpain.2010.01.005
Ruthes AC, Carbonero ER, Córdova MM, Baggio CH, Sassaki GL, Gorin PAJ, et al. Fucomannogalactan and glucan from mushroom Amanita muscaria: Structure and inflammatory pain inhibition. Carbohydr Polym. 2013;98(1):761-9. https://doi.org/10.1016/j.carbpol.2013.06.061
Abreu H, Simas FF, Smiderle FR, Sovrani V, Dallazen JL, Ferreira DM, et al. Gelling functional property, anti-inflammatory and antinociceptive bioactivities of β-D-glucan from the edible mushroom Pholiota nameko. Int J Biol Macromol. 2019;122:1128-35. http://doi.org/10.1016/j.ijbiomac.2018.09.062
Silveira MLL, Smiderle FR, Agostini F, Pereira EM, Bonatti-Chaves M, Wisbeck E, et al. Exopolysaccharide produced by Pleurotus sajor-caju: Its chemical structure and anti-inflammatory activity. Int J Biol Macromol. 2015;75:90-6. https://doi.org/10.1016/j.ijbiomac.2015.01.023
Jen CI, Su CH, Lai MN, Ng LT. Comparative anti-inflammatory characterization of selected fungal and plant water soluble polysaccharides. Food Sci Technol Res. 2021;27(3):453-62. https://doi.org/10.3136/fstr.27.453
Yelithao K, Surayot U, Lee C, Palanisamy S, Prabhu NM, Lee J, et al. Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune. Carbohydr Polym. 2019;218:37-45. https://doi.org/10.1016/j.carbpol.2019.04.057
Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017;9(6):7204-18. https://doi.org/10.18632/oncotarget.23208
Murphy EJ, Rezoagli E, Pogue R, Simonassi-Paiva B, Abidin IIZ, Fehrenbach GW, et al. Immunomodulatory activity of β-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions. Sci Total Environment. 2022;809:152177. https://doi.org/10.1016/j.scitotenv.2021.152177
Baggio CH, Freitas CS, Marcon R, Werner MFP, Rae GA, Smiderle FR, et al. Antinociception of β-d-glucan from Pleurotus pulmonarius is possibly related to protein kinase C inhibition. Int J Biol Macromol. 2012;50(3):872-7. https://doi.org/10.1016/j.ijbiomac.2011.10.023