Occupational exposure to isoflurane impairs liver redox status in mice
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Abstract
Introdução: O isoflurano, amplamente utilizado em ambientes médicos e científicos, desempenha um papel significativo na geração de gases anestésicos residuais. A exposição ocupacional a este anestésico pode resultar em graves prejuízos à saúde devido à exposição crônica, principalmente no que diz respeito ao impacto oxidativo. O fígado, sendo o principal órgão responsável pelo metabolismo deste anestésico, é um alvo chave para estes efeitos adversos. Objetivo: Avaliar os efeitos da exposição ocupacional crônica ao isoflurano sobre o estado redox hepático de camundongos e investigar se a interrupção da exposição pode restaurar o equilíbrio oxidativo. Métodos: Os ratos foram expostos a 50 ppm de isoflurano durante 5 horas por dia durante um período de 30 dias. Após a exposição, eles foram sacrificados no primeiro dia após o término da exposição (G2) ou 20 dias após a última exposição (G3) e comparados ao grupo controle (G1). Suas amostras de tecido hepático foram submetidas a análises bioquímicas, avaliando-se a concentração de espécies reativas de oxigênio e substâncias reativas ao ácido tiobarbitúrico, bem como a atividade das enzimas catalase e superóxido dismutase. Resultados: A exposição ocupacional ao isoflurano pode afetar a atividade de enzimas antioxidantes no tecido hepático. Os indivíduos expostos apresentaram redução da atividade da catalase, associada a um aumento tardio e não fisiológico da atividade da superóxido dismutase. Conclusão: O isoflurano em concentrações supostamente seguras perturba o estado redox hepático dos indivíduos expostos, e a interrupção da exposição não garante o retorno ao equilíbrio oxidativo.
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