Exposição ocupacional ao isoflurano prejudica status redox hepático em camundongos
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Introdução: O isoflurano, amplamente utilizado em ambiente médico e científico, desempenha um papel significativo na geração de resíduos de gases anestésicos. Trabalhadores expostos ocupacionalmente a esse anestésico podem sofrer sérios prejuízos à saúde decorrentes da exposição crônica, especialmente no que tange ao impacto oxidativo. O fígado, principal responsável pela metabolização deste anestésico, é um alvo chave para estes efeitos adversos. Objetivo: Avaliar os efeitos da exposição ocupacional crônica ao isoflurano no status redox hepático de camundongos, e investigar se a interrupção da exposição é capaz de reestabelecer o equilíbrio oxidativo. Métodos: Os camundongos foram expostos a 50 ppm de isoflurano, 5 horas/dia, durante um período de 30 dias. Após a exposição, foram eutanasiados em diferentes intervalos (G2 - no primeiro dia após a interrupção da exposição; G3 - 20 dias após a última exposição) e comparados ao grupo controle (G1). Suas amostras de tecido hepático foram submetidas a análises bioquímicas; concentração de espécies reativas de oxigênio e de substâncias reativas ao ácido tiobarbitúrico, bem como atividade das enzimas catalase e superoxidodismutase foram avaliadas. Resultados: A exposição ocupacional ao isoflurano é capaz de afetar a atividade das enzimas antioxidantes no tecido hepático. Indivíduos expostos apresentam redução na atividade de catalase, associada ao incremento tardio e antifisiológico na atividade de superoxidodismutase. Conclusão: O isoflurano em concentrações seguras perturba o status redox hepático dos indivíduos expostos, e a interrupção da exposição não garante o retorno ao equilíbrio oxidativo.
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